Implication of CYP24A1 Splicing in Breast Cancer

ISSN: 1875-5992 (Online)
ISSN: 1871-5206 (Print)

Volume 17, 14 Issues, 2017

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Anti-Cancer Agents in Medicinal Chemistry

Formerly: Current Medicinal Chemistry - Anti-Cancer Agents

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Michelle Prudhomme
Institut de Chimie de Clermont-Ferrand
Université Clermont Auvergne

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Implication of CYP24A1 Splicing in Breast Cancer

Anti-Cancer Agents in Medicinal Chemistry, 14(1): 109-114.

Author(s): Chimi Scheible, Marc Thill, Sascha Baum, Erich Solomayer and Michael Friedrich.

Affiliation: Klinik für Frauenheilkunde und Geburtshilfe, Helios-Klinikum Krefeld, Lutherplatz 40, D-47805 Krefeld, Germany.


The expression of CYP27B1 or vitamin D 1α-hydroxylase (1α-OHase) and CYP24A1 in specific tissues may act as the central part between 25-hydroxyvitamin D [25(OH)D] serum levels and the anticancer effects of1α,25-dihydroxyvitamin D [1α,25(OH)2D3],alternative splicing of these enzymes may affect their biological functions. Here, we describe the expression of CYP24A1 and its splicing variants detected in breast cells and tissues.

Manifestation of CYP24A1 mRNA was measured by RT-PCR followed by western blot analysis for protein expression.

In MCF-7 cells, the expression of CYP24A1 protein was reduced by about 57% compared to MCF-10F cells. Western blot analysis revealed a signal band at 56 kDa, with additional bands detected at 42 and 44 kDa.

The expression of CYP24A1 mRNA was reduced by about 58% in breast cancer tissues. We found only one signal in the benign tissues at 56 kDa in western blot, whereas in malignant tissue, an additional band was detected at 40kDa.

Our results suggest that alternative splicing of CYP24A1 may lead to a catalytically dysfunctional enzyme.


Vitamin D<sub>3</sub>, 24-hydroxylase, splicing variants, MCF-7, MCF-10, breast tissue.

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Article Details

Volume: 14
Issue Number: 1
First Page: 109
Last Page: 114
Page Count: 6
DOI: 10.2174/18715206113139990311
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