Protein Content Analysis and Antimicrobial Activity of the Crude Venom of Montivipera bornmuelleri; a Viper from Lebanon
Claudine Accary, Souad Hraoui-Bloquet, Monzer Hamze, Yassine Mallem, Fawaz El Omar, Jean-Marc Sabatier, Jean-Claude Desfontis and Ziad FajlounAffiliation:
Azm Center for Research in Biotechnology and its Applications, Doctoral School for Sciences and Technology or Faculty of Sciences III, Lebanese University, El Mittein Street, Tripoli, Lebanon.
AbstractViperidae snakes venoms represent a source of efficient bioactive components that have already led to the development of several new drugs. In this work, we analyzed the protein content of the Montivipera bornmuelleri crude venom using LC-ESI-MS, sephadex G-75 gel filtration and SDS-PAGE and demonstrated the presence of proteins with molecular masses corresponding to metalloprotease III, serine-protease and PLA2 in three fractions collected after gel filtration. Equally, we examined the antimicrobial effect of the venom that showed an important potency, as bactericidal agent, based on MBC and MIC values obtained, against Staphylococcus aureus and Morganella morganii bacteria. However, no activity was registered against Enterococcus faecalis, being the most resistant bacteria, neither against Aspergillus flavus and Penicillium digitatum fungal. Furthermore, on eleven other bacterial strains and the Candida albicans fungus, the venom has shown an intermediate efficacy by slightly reducing the growth. Our data concerning the Montivipera bornmuelleri venom give evidence of a rich and complex content aiding the exploration of new bioactive molecules for biopharmaceuticals purposes.
Antibacterial and antifungal activities, crude venom, Montivipera bornmuelleri, proteome analysis.
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