Identification of L-Amino Acid Oxidase (Mb-LAAO) with Antibacterial Activity in the Venom of Montivipera bornmuelleri, a Viper from Lebanon

ISSN: 2212-3989 (Online)
ISSN: 1871-5265 (Print)


Volume 14, 3 Issues, 2014


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Infectious Disorders - Drug Targets

Formerly: Current Drug Targets - Infectious Disorders

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Editor-in-Chief:
Jean-Marc Sabatier
Laboratoire ERT 62 'Ingénierie des peptides à visée thérapeutique'
Université de la Méditerranée
Faculté de Médecine Nord
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13916 - MARSEILLE, Cedex 20
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Identification of L-Amino Acid Oxidase (Mb-LAAO) with Antibacterial Activity in the Venom of Montivipera bornmuelleri, a Viper from Lebanon

Author(s): Mohamad Rima, Claudine Accary, Katia Haddad, Riyad Sadek, Souad Hraoui-Bloquet, Jean C. Desfontis and Ziad Fajloun

Affiliation: Azm Center for Research in Biotechnology and its Applications, Doctoral School of Science and Technology, Lebanese University, El Mittein Street, Tripoli, Lebanon.

Abstract

The L-amino acid oxidase (LAAO) is a multifunctional enzyme, able to partake in different activities including antibacterial activity. In this study, a novel LAAO (Mb-LAAO) was isolated from the venom of M. bornmuelleri snake using size exclusion chromatography followed by RP-HPLC and partially characterized. However, the molecular weight of the Mb-LAAO determined by ESI-MS and SDS-PAGE was 59 960.4 Da. Once the enzymatic activity test confirming the enzyme’s identity (transformation of L-leucine) was done, the Mb-LAAO was evaluated for its antibacterial activity against Gram-negative bacteria. It showed a remarkable effect against M. morganii and K. pneumoniae. Moreover, no cytotoxic activity was observed for Mb-LAAO against human erythrocytes arguing for an exploration of its pharmaceutical interest.

Keywords: Antibacterial effect, LAAO activity, L-amino acid oxidase, LC-ESI-MS analysis, Montivipera bornmuelleri.

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Article Details

Volume: 13
Issue Number: 5
First Page: 337
Last Page: 343
Page Count: 7
DOI: 10.2174/187152651305140403122334
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