Qi-Shao-Shuang-Gan Prevents Adhesion between Leukocytes and Inflammatory Endothelial Cells
Current Signal Transduction Therapy,
Xinghua Gao, Difei Bian, Xianxiang Xu, Rong Pan, Yufeng Xia, Side Jiang and Yue DaiAffiliation:
Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, China.
AbstractOur previous studies demonstrated that Qi-Shao-Shuang-Gan (QSSG), a combination of Astragalus membranaceus saponins (SAM) with Paeonia lactiflora glycosides (GPL), possessed protective effects against sepsis in mice. The present study aimed to explore its underlying mechanisms with a focus on the adhesion between leukocytes and inflammatory endothelial cells. Our results showed that QSSG (10, 30, 100 μg/ml) concentration-dependently inhibited the adhesion between leukocytes and lipopolysaccharide (LPS)-stimulated human umbilical vein endothelial cells (HUVECs), and downregulated the expressions of endothelial adhesion molecules such as E-selectin, P-selectin, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in HUVECs. It also suppresssed the phosphorylations of JNK, ERK and p38 MAPK, as well as the activation of NF-κB in HUVECs. The findings revealed that QSSG was able to prevent the adhesion between leukocytes and endothelial cells, which supported a plausible explanation for the antiseptic effects of QSSG.
QSSG, adhesion, adhesion molecules, antiseptic effect, endothelial cells, leukocytes.
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