VEGF, BMP-7, Matrigel<sup>TM</sup>, Hyaluronic Acid, In Vitro Cultured Chondrocytes and Trephination for Healing of the Avascular Portion of the Meniscus. An Experimental Study in Sheep

ISSN: 2212-3946 (Online)
ISSN: 1574-888X (Print)


Volume 9, 6 Issues, 2014


Download PDF Flyer




Current Stem Cell Research & Therapy

Aims & ScopeAbstracted/Indexed in


Submit Abstracts Online Submit Manuscripts Online

Editor-in-Chief:
Anthony Atala
Wake Forest University School of Medicine,
Medical Center Boulevard
Winston Salem, NC 27157
USA


View Full Editorial Board

Subscribe Purchase Articles Order Reprints

Current: 2.861
5 - Year: 3.00

VEGF, BMP-7, MatrigelTM, Hyaluronic Acid, In Vitro Cultured Chondrocytes and Trephination for Healing of the Avascular Portion of the Meniscus. An Experimental Study in Sheep

Author(s): Francisco Forriol, Umile Giuseppe Longo, Julio Duart, Purification Ripalda, Javier Vaquero, Mattia Loppini, Giovanni Romeo, Stefano Campi, Wasim S. Khan, Andrea O. Muda and Vincenzo Denaro

Affiliation: University Campus Bio- Medico of Rome, Italy.

Abstract

Objective: To evaluate the effects of VEGF, BMP-7, MatrigelTM, hyaluronic acid, in vitro cultured chondrocytes and trephination to promote and enhance the healing process of avascular meniscal tears in an animal model. Methods: A longitudinal tear was produced in the inner avascular part of the meniscus of 24 sheeps. Each tear was treated with trephination technique and suture. The animals were divided into 6 groups to receive a different treatment: control (I); VEGF, BMP-7, MatrigelTM, hyaluronic acid, in vitro cultured chondrocytes. At 8 weeks from surgery, meniscal samples were explanted and analyzed by histology, immunohistochemistry, and histomorphometry. Results: At the histological examination, Group IV and VI showed a partial closure of the meniscal lesion, whereas Group I, II, III, and V did not show any evidence of healing. In the group IV, the healed tissue represented the 22.95% of the lesion area. In the group VI, the healed tissue represented the 43.75% of the lesion area. Conclusions: Autologous chondrocytes and BMP-7 associated with trephination and suture techniques enhanced healing process of meniscal tears in the avascular inner third of the meniscus in ovine model.

Keywords: BMP-7, cell-therapy, healing, growth factors, meniscus, repair.

Purchase Online Order Reprints Order Eprints Rights and Permissions

  
  



Article Details

Volume: 10
Issue Number: 1
First Page: 69
Last Page: 76
Page Count: 8
DOI: 10.2174/1574888X09666140710115356
Advertisement

Related Journals




Webmaster Contact: urooj@benthamscience.org Copyright © 2014 Bentham Science