Highly Sensitive and Simple Validated Ultra-performance Liquid Chromatography/ Tandem Mass Spectrometry Method for the Determination of Cinacalcet in Human Plasma
Tanveer A. Wani, Nasr Y. Khalil, Ibrahim Darwish, Muzaffar Iqbal and Ahmed H. BakheitAffiliation:
Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
AbstractWith the objective of decreasing analysis time and retaining good efficiency (UPLC-MS/MS) is an outstanding analytical approach for speedy biomedical analysis. The aim of this study was to develop validate a simple, rapid, sensitive and specific UPLC-MS/MS method for quantification of cinacalcet, a calcimimetic agent, which acts on a calcium sensing receptor of the parathyroid gland in human plasma. After following a liquid–liquid extraction procedure with diethyl ether–dichloromethane (70:30, v/v), cinacalcet (CIN) and internal standard (IS) abiraterone with C18 Acquity UPLC BEHTM column. m/z 358.07 > 155.0 and m/z 350.1 > 156.0 where the ion transitions recorded in the positive ion multiple reaction monitoring mode for cinacalcet for IS. The mobile phase consisted of acetonitrile: 10 mM ammonium acetate: formic acid (90:10:0.1% v/v/v) with a flow rate of 0.4 mL/min. The assay exhibited a linear dynamic range of 0.2–100 ng/mL for cinacalcet in human plasma with good correlation coefficient (IQ 0.995) and with an LOQ of 1 ng/mL. The intra- and inter-assay precisions were satisfactory; the relative standard deviations did not exceed 6.98 %. This method was simple, rapid and highly sensitive, hence could be used for analysis of cinacalcet in human plasma.
Cinacalcet, UPLC, LC-MS/MS, pharmacokinetic study, human plasma.
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