Affiliation: College of Life Science, Zhejiang Chinese medical University, Hangzhou, 310053, China.
Atherosclerosis is considered a chronic inflammatory disease and injury to vascular endothelial cells is an important event at the onset of its pathogenesis. Oxidative stress is regarded as a pivotal pathogenic factor in the development of endothelial cell injury and apoptosis. Tanshinone IIA is considered the main active lipophilic component of the traditional Chinese medicine Danshen. It has been demonstrated that Tanshinone IIA protects endothelial cells from oxidative stress-triggered injury and apoptosis, but many of its anti-oxidant and anti-apoptotic mechanisms still remain to be elucidated.
In this paper, our results show that HUVECs incubated with 1.1mM H2O2 for 12h had significantly decreased the viability of endothelial cells, which was accompanied with obviously cell apoptosis. However, treatment with Tanshinone IIA (8 mM, 24h) resulted in a significant resistance to H2O2-induced apoptosis. We used 2D-DIGE (Difference in Gel Electrophoresis) analysis followed by MALDI-TOF-TOF-MS to identify the proteins differentially expressed after human umbilical vein endothelial cells (HUVECs) were protected with Tanshinone IIA against oxidative damage by hydrogen peroxide. There were various differently expressed proteins, we picked out 32 proteins to analysis, and discovered that Tanshinone IIA could protect endothelial cells by its anti-inflammatory or antioxidant effects, regulating proliferation and differentiation, maintaining the cytoskeleton, and maintaining intracellular calcium homeostasis.