Covalent Immobilization of α -Glucosidase Enzyme onto Amine Functionalized Multi-walled Carbon Nanotubes
M. Mohiuddin, D. Arbain, A.K.M. Shafiqul Islam, M. Rahman, M.S. Ahmad and M.N. AhmadAffiliation:
School of Bioprocess Engineering, Centre of Excellence for Advanced Sensor Technology, Universiti Malaysia Perlis, 01000, Kangar, Perlis, Malaysia.
Abstractα -glucosidase (AG) enzyme was immobilized onto amine functionalized multi-walled carbon nanotubes (MWCNTs-NH2) by treatment with cross linkers, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) via amide bonding. The immobilized AG was characterized by Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM). The performance of the electrode was evaluated by Spectrophotometric method and cyclic voltammetric (CV) method using p-nitrophenyl-α -D-glucopyranoside (PNPG) as a substrate. The immobilized AG enzyme retains about 84.16% activity of its initial response even after 30 days when stored at 4°C.
Cyclic voltammetry, functionalization, α -glucosidase, immobilization.
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