A Possible Role for Interleukin 37 in the Pathogenesis of Behcet's Disease

ISSN: 1875-5666 (Online)
ISSN: 1566-5240 (Print)


Volume 14, 10 Issues, 2014


Download PDF Flyer




Current Molecular Medicine

Aims & ScopeAbstracted/Indexed in

Ranking and Category:
  • 31st of 122 in Medicine, Research & Experimental

Submit Abstracts Online Submit Manuscripts Online

Editor-in-Chief:
David W. Li
College of Medicine
University of Nebraska Medical Center
Omaha, NE
USA


View Full Editorial Board

Subscribe Purchase Articles Order Reprints

Current: 3.612
5 - Year: 4.159

A Possible Role for Interleukin 37 in the Pathogenesis of Behcet's Disease

Author(s): Z. Ye, C. Wang, A. Kijlstra, X. Zhou and P. Yang

Affiliation: The First Affiliated Hospital of Chongqing Medical University, Youyi Road 1, Chongqing, 400016, P.R. China.

Abstract

Interleukin 37 has been found to play a significant regulatory role in the innate immune response. It is not yet known whether IL-37 has also been involved in the development of Behcet’s disease (BD), a chronic systemic inflammatory disease. To examine the role of IL-37 in the pathogenesis of BD, a number of experiments were performed. IL-37 expression in peripheral blood mononuclear cells (PBMCs) from BD patients and normal controls was measured by RT-PCR and flow cytometry. Monocyte-derived Dendritic Cells (DCs) were cultured with or without IL-37 and levels of cytokines in the culture supernatants were measured by ELISA. The DC surface markers, reactive oxygen species (ROS) production and mitogen-activated protein kinase (MAPK) activation were measured by flow cytometry. The effect of IL-37-treated DCs on the development of CD4+ T cells was measured by ELISA and flow cytometry. The results show that both IL-37 mRNA level and protein expression were significantly decreased in PBMCs from active BD patients compared to normal controls. DCs stimulated with rIL-37 showed a decreased expression of IL-6, IL-1β and TNF-α, and a higher production of IL-27. rIL-37 significantly inhibited the production of ROS by DCs and reduced the activation of ERK1/2, JNK and P38 MAPK in DCs. rIL-37-treated DCs remarkably inhibited Th17 and Th1 cell responses as compared to control DCs. rIL-37 did not affect the expression of DC surface markers (CD40, CD86, CD80 and HLA-DR) or IL-10 production by DCs. We conclude that a decreased IL-37 expression in active BD patients may trigger the production of pro-inflammatory cytokines and ROS in association with activation of Th1 and Th17 cells by DCs.

Keywords: Behcet's disease, cytokine, dendritic cells, IL-37, inflammatory response, MAPK family, reactive oxygen species, uveitis.

Purchase Online Order Reprints Order Eprints Rights and Permissions

  
  



Article Details

Volume: 14
Issue Number: 4
First Page: 535
Last Page: 542
Page Count: 8
DOI: 10.2174/1566524014666140414210831
Advertisement

Related Journals




Webmaster Contact: urooj@benthamscience.org Copyright © 2014 Bentham Science