Fabrication of Split-Luciferase Complementation Assays for Molecular Imaging

ISSN: 2211-5544 (Online)
ISSN: 2211-5552 (Print)

Volume 4, 2 Issues, 2015

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Current Molecular Imaging

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Abass Alavi
Department of Radiology/Nuclear Medicine
Hospital of the University of Pennsylvania
Philadelphia, PA 19104
Email: abass.alavi@uphs.upenn.edu

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Fabrication of Split-Luciferase Complementation Assays for Molecular Imaging

Current Molecular Imaging, 2(2): 148-157.

Author(s): Sung Bae Kim and Takeaki Ozawa.

Affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Japan.


Recent revolutionary developments in luciferase engineering technologies now allow researchers to carry out quantitative imaging of molecular dynamics and cell signaling in living subjects, the technology of which includes protein-fragment complementation assay (PCA) and intein-mediated protein splicing assay (PSA). These technologies are functionally optimized with specific marine and beetle luciferases exerting designed properties and functionalities. This review overviews how to fabricate such bioluminescent tools and technologies of reconstituting split-luciferases, providing an excellent platform for today’s molecular imaging.


Bioluminescence, luciferase, protein-fragment complementation assay (PCA), single-chain probes.

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Article Details

Volume: 2
Issue Number: 2
First Page: 148
Last Page: 157
Page Count: 10
DOI: 10.2174/22115552113029990001

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