Genotypic and Phenotypic Comparison of Enteroaggregative Escherichia coli Isolates from HIV-Positive and Non-HIV Diarrheal Samples

ISSN: 1873-4251 (Online)
ISSN: 1570-162X (Print)


Volume 12, 6 Issues, 2014


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Genotypic and Phenotypic Comparison of Enteroaggregative Escherichia coli Isolates from HIV-Positive and Non-HIV Diarrheal Samples

Author(s): Anis Jafari, Ebrahim Shafaei, Mana Oloomi, Mohammad-Reza Aghasadeghi and Saeid Bouzari

Affiliation: Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran.

Abstract

Enteroaggregative Escherichia coli (EAEC) have been isolated from both HIV-positive and non-HIV diarrheal samples. In this study a collection of 18 isolates from these two groups were compared for biofilm formation and antibiotic resistance and for the presence of 14 virulence-related genes. All the HIV-positive and over 66% of the non- HIV strains were PCR-negative for adhesion-related sequences indicating that as yet unknown adhesins may play a role. However, despite some variations, the prevalence rate of the virulence-related genes was not significantly different in the two groups. HIV-positive isolates were biofilm producer but only a single weak biofilm former was observed among the non-HIV strains. The rate of resistance to most of the antibiotics used was higher among the HIV-positive group than the non-HIV isolates, but was significantly higher for amoxicillin-calvulanic acid (100%) and nalidixic acid (55.5%). Pulse field gel electrophoresis of the isolates produced 17 unique profiles reflecting the exiting heterogeneity of the isolates.

Keywords: Antibiotic resistance, biofilm, Enteroaggregative Escherichia coli, EAEC, HIV-positive diarrhea, non-HIV diarrhea, virulence-related genes.

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Article Details

Volume: 11
Issue Number: 8
First Page: 635
Last Page: 641
Page Count: 7
DOI: 10.2174/1570162X12666140207161000
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