Comparing Inhibitory Effect of Tramadol on Catalase of Pseudomonas aeruginosa and Mouse Liver

ISSN: 1875-6662 (Online)
ISSN: 1573-4080 (Print)

Volume 13, 3 Issues, 2017

Download PDF Flyer

Current Enzyme Inhibition

This journal supports open access

Aims & ScopeAbstracted/Indexed in

Submit Abstracts Online Submit Manuscripts Online

Professor Claudiu T. Supuran
University of Florence, Neurofarba Department
Sesto Fiorentino (Firenze)

View Full Editorial Board

Subscribe Purchase Articles Order Reprints

Research Article

Comparing Inhibitory Effect of Tramadol on Catalase of Pseudomonas aeruginosa and Mouse Liver

Current Enzyme Inhibition, 10(1): 53-57.

Author(s): Dariush Minai-Tehrani, Imana S. Ashrafi, Mina K. Mohammadi, Zahra S. Damavandifar, Elmira R. Zonouz and Tahmineh E Pirshahed.

Affiliation: BioResearch Lab, Faculty of Biological Sciences, Shahid Beheshti University, G.C. Iran.


Tramadol is an analgesic drug that binds to specific opioid receptors. It may contribute to the inhibition of neuronal re-uptake of noradrenaline. Catalase is a key enzyme for degrading H2O2 in cells and has various isoforms with different structures and kinetics properties. In this research, the effect of tramadol on the activity of catalase of Pseudomonas and mouse liver was investigated and compared. Tramadol could inhibit Pseudomonas catalase with mixed inhibition while inhibiting mouse liver catalase in a non-competitive manner. The Ki and IC50 values were determined as 0.45 and 1.5 mM for Pseudomonas and 2.8 and 2.5 mM for mouse liver, respectively. SDS-PAGE of partially purified catalases determined different molecular weights for Pseudomonas (Mw 54 kDa) and mouse liver (61 kDa).


Bacteria, Drug, Enzyme, Inhibition, Mouse liver.

Download Free Order Reprints Order Eprints Rights and Permissions

Article Details

Volume: 10
Issue Number: 1
First Page: 53
Last Page: 57
Page Count: 5
DOI: 10.2174/15734080113099990002

Related Journals

Webmaster Contact: Copyright © 2016 Bentham Science