Preparation and Characterization of Methylene blue Nanoparticles for Alzheimer's Disease and Other Tauopathies

ISSN: 1875-5704 (Online)
ISSN: 1567-2018 (Print)

Volume 14, 8 Issues, 2017

Download PDF Flyer

Current Drug Delivery

This journal supports open access

Aims & ScopeAbstracted/Indexed in

Submit Abstracts Online Submit Manuscripts Online

Istvan Toth
School of Pharmacy,University of Queensland
Brisbane, 4072

View Full Editorial Board

Subscribe Purchase Articles Order Reprints

Current: 1.446

Preparation and Characterization of Methylene blue Nanoparticles for Alzheimer's Disease and Other Tauopathies

Current Drug Delivery, 11(4): 541-550.

Author(s): Umesh K. Jinwal, Anastasia Groshev, Juan Zhang, Aditya Grover and Vijaykumar B Sutariya.

Affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, University of South Florida, Tampa, FL 33612, USA.


Methylene blue (MB) has been shown to slow down the progression of the Alzheimer’s disease (AD) and other tauopathies; however distribution of MB into the brain is limited due its high hydrophilicity. In this study, we aimed to prepare novel hydrophobic glutathione coated PLGA nanoparticles to improve bioavailability of MB in the brain. Glutathione coated poly-(lactide-co-glycolide) (PLGA-b-PEG) nanoparticles (NPs) were prepared and tested in two different cell culture models of AD expressing microtubule associated protein tau (tau). The NPs showed a particle size averaging 136.5±4.4nm, which is suitable for the blood brain barrier (BBB) permeation. The in vitro release profile of the NPs exhibited no initial burst release and showed sustained drug release for up to 144 hours. Interestingly, treatment of newly formulated MB-NPs showed a potent reduction in both endogenous and over expressed tau protein levels in human neuroblastoma SHSY-5Y cells expressing endogenous tau and transfected HeLa cells over-expressing tau protein, respectively. Furthermore, in vitro BBB TranswellTM study showed significantly higher permeation of MB-NP compared to the MB solution through the co culture of rat brain endothelial 4 (RBE4) and C6 astrocytoma cells (p<0.05). The proposed MB loaded nanoparticles could provide a more effective treatment option for AD and many other related disorders.


Alzheimer&#39;s disease, blood brain barrier, brain targeted delivery, drug delivery, Methylene blue, PLGA nanoparticles.

Purchase Online Order Reprints Order Eprints Rights and Permissions

Article Details

Volume: 11
Issue Number: 4
First Page: 541
Last Page: 550
Page Count: 10
DOI: 10.2174/1567201810666131113102037
Price: $58
14th annual Controlled Release Delivery

Related Journals

Webmaster Contact: Copyright © 2016 Bentham Science