A Method for Measuring Difference in Activity of Phenolic and Non- Phenolic Groups Through 2,2` Azino-Bis-(3-Ethyl-Benzothiazoline-6- Sulphonate) Radical Cation
Nusrat Masood and Suaib LuqmanAffiliation:
Molecular Bioprospection Department, Biotechnology Division, CSIR-Central Institute of Medicinal & Aromatic Plants, Lucknow-226015, India.
AbstractA 2, 2`azino-bis-(3-ethyl-benzothiazoline-6-sulphonate) radical cation-based method was optimized in 96 well plates to evaluate the comparative scavenging potential of phenolic and non-phenolic group of molecules with respect to control. The interactions of these molecules with ABTS radical cation were quantified on the basis of their relative influence on the bleaching of a bluish-green color complex in a structure- and dose-dependent manner. Experimentally, the developed assay provided evidence that phenolic molecules are more reactive with ABTS radical than non-phenolic compounds because of their resonance and hyper-conjugation effects.
2, 2`azino-bis-(3-ethyl-benzothiazoline-6-sulphonate), butylated hydroxytoluene, butylated hydroxyanisole, tertiary butylhydroquinone, trolox.
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