Stability Indicating Rapid Resolution LC Method for Determination of Valaciclovir Hydrochloride and its Application on Determination of its Active Metabolite Aciclovir in Human Plasma

ISSN: 2213-2414 (Online)
ISSN: 2213-2406 (Print)


Volume 1, 2 Issues, 2014






Current Chromatography

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Editor-in-Chief:
Pavel N. Nesterenko
Australian Centre for Research on Separation Science (ACROSS)
School of Chemistry, University of Tasmania
Private Bag 75
Hobart, TAS 7001
Australia


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Stability Indicating Rapid Resolution LC Method for Determination of Valaciclovir Hydrochloride and its Application on Determination of its Active Metabolite Aciclovir in Human Plasma

Author(s): Lobna A. El-aziz Hussein, Maha Farouk A. El-ghany and Marwa Soliman

Affiliation: National Organization of Drug Control and Research (NODCAR), 51 Wezzart Elzeraa Street, Elagouza, Giza, Egypt.

Abstract

A sensitive, precise and environmentally friendly stability indicating Rapid Resolution Liquid Chromatographic (RRLC) method for the determination of valaciclovir HCl in presence of its degradation products was developed and validated. An isocratic separation was achieved using Zorbax SB phenyl column with flow rate of 0.3 mL min-1 and UV detection at 254 nm. The mobile phase was a mixture of 0.01M n-tetrabutyl ammonium hydrogen sulfate (pH 2.5, adjusted using 0.1M NaOH) and methanol in the ratio of (95:5, v/v). The stability of valaciclovir HCl has been studied under different stress conditions. The acid-and alkaline-hydrolysis products were identified and were suggested to be guanine and aciclovir, respectively. Complete separation of valaciclovir hydrochloride and its degradation products was achieved in overall run time of approximately 6.0 min with low mobile phase consumption and waste production (1.8ml/run). The method was linear over the range of 0.4 µg mL-1 to 40.0 µg mL-1 (r=0.9999) with limit of detection and quantitation of 0.10 µg mL-1 and 0.36 µg mL-1, respectively. The high efficiency and unique selectivity of developed method enabled its adoption for determination of aciclovir, the active metabolite of valaciclovir, in spiked human plasma.

Keywords: Liquid Chromatography, stability indicating, plasma, valaciclovir and aciclovir.

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Article Details

Volume: 1
Issue Number: 2
First Page: 151
Last Page: 159
Page Count: 9
DOI: 10.2174/2213240601666140303233836
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