Chiral High-Speed Counter-Current Chromatography: Future Strategies for Chiral Selector Development

ISSN: 2213-2414 (Online)
ISSN: 2213-2406 (Print)


Volume 1, 2 Issues, 2014






Current Chromatography

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Editor-in-Chief:
Pavel N. Nesterenko
Australian Centre for Research on Separation Science (ACROSS)
School of Chemistry, University of Tasmania
Private Bag 75
Hobart, TAS 7001
Australia


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Chiral High-Speed Counter-Current Chromatography: Future Strategies for Chiral Selector Development

Author(s): Ying Ma and Yoichiro Ito

Affiliation: Laboratory of Bioseparation Technology, Biochemistry and Biophysics Center, NHLBI, NIH, Bethesda, MD 20892, USA

Abstract

In conventional high-performance liquid chromatography, chiral separations are performed by chiral column with a chiral selector (CS) chemically bonded to the solid support. In contrast, high-speed counter-current chromatography (HSCCC) performs chiral separations by dissolving CS in the liquid stationary phase. During the past two decades, several CSs were developed to successfully carry out chiral HSCCC which include N-dodecanoyl-L-proline-3,5- dimethylanilide, β-cyclodextrin derivatives, vancomycin, cinchona alkaloid derivatives, cellulose and amylose derivatives, tartaric acid derivatives, etc. Compared to HPLC which uses over hundred different kinds of CSs, the number of CSs effectively used in HSCCC is limited to several compounds. This may be due to the violent molecular movement of CS dissolved in the liquid stationary phase which reduces chiral selectivity based on steric affinity. Future development strategy of CS for HSCCC proposed here is to suppress the molecular movement of the CS in the liquid stationary phase by the following three ways: 1) using viscous stationary phase such as aqueous-aqueous polymer phase system; 2) attaching a long hydrophobic chain to the asymmetric carbon, or 3) chemically bonding CS onto hydrophobic small particles such as carbon nanotubes, gold colloidal particles, and submicron silica particles.

Keywords: Chiral separation, high-speed counter-current chromatography, chiral selector, steric interaction.

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Article Details

Volume: 1
Issue Number: 1
First Page: 69
Last Page: 80
Page Count: 12
DOI: 10.2174/22132406113099990001
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