Engineering Lipases for Enhanced Catalysis
Bhavya Ravi, Ujjwal Banerjee, Sandhya Mehrotra and Rajesh MehrotraAffiliation:
Department of Biological Sciences, Chamber # 3222K Faculty Division III BITS, Pilani, 3222Q.
AbstractLipases are versatile biocatalysts that catalyze a plethora of reactions such as hydrolysis, esterification, transesterification, aminolysis and acidolysis, making them one of the most sought biocatalysts for industrial applications. However, their widespread use in industry is hampered by problems arising mainly due to loss of activity, thermal denaturation, deactivation by organic solvents etc., which affect reaction yields and lead to impaired enzyme recyclability and reusability for successive runs. Methods such as enzyme immobilization, reaction engineering and molecular biology approaches are being used to alleviate these impediments. Lipase bio-imprinting and molecular biology techniques such as rational protein design and directed evolution have made it possible to engineer lipases having superior properties and selectivities, greatly enhancing their activities and operational stability thereby improving reaction yields. This review focuses on the applicability of the above-mentioned strategies to create tailor-designed lipase enzymes to suit specific reactions needs with an emphasis on the transesterification reaction to produce biodiesel.
Bio-imprinting, directed evolution, error-prone PCR, Rational Protein Design, site-directed mutagenesis.
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