Overexpression of XlnR Regulated Hemicellulase-Encoding Genes in Aspergillus Vadensis

ISSN: 2211-551X (Online)
ISSN: 2211-5501 (Print)


Volume 3, 4 Issues, 2014


Download PDF Flyer




Current Biotechnology

Aims & ScopeAbstracted/Indexed in


Submit Abstracts Online Submit Manuscripts Online

Editor-in-Chief:
Pabulo Henrique Rampelotto
Interdisciplinary Center for Biotechnology Research
Federal University of Pampa
São Gabriel, RS
Brazil


View Full Editorial Board

Subscribe Purchase Articles Order Reprints


Overexpression of XlnR Regulated Hemicellulase-Encoding Genes in Aspergillus Vadensis

Author(s): Ourdia Bouzid and Ronald P. de Vries

Affiliation: CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.

Abstract

Aspergillus vadensis is a close relative of Aspergillus niger and is particularly interesting for hemicellulase production as it does not produce extracellular proteases. The main regulator driving hemicellulase production in Aspergilli and other fungi is XlnR and overexpression of this regulator leads to increased hemicellulase levels in some species. To increase hemicellulase production in A. vadensis strains were generated that contain multiple copies of two XlnR target genes, xlnD and faeA, encoding β-xylosidase and feruloyl esterase A respectively, and/or with multiple copies of xlnR. The strains were compared with respect to β-xylosidase and feruloyl esterase activity, respectively, to determine the effect of overexpression of xlnR in comparison to overexpression of the target genes (faeA, xlnD). The results demonstrate that insertion of additional copies of faeA and xlnD had a much larger effect on the corresponding activities than additional copies of xlnR in A. vadensis, suggesting that the amount of XlnR present in A. vadensis is sufficient to activate additional copies of the target genes without much effect on the production of other XlnR-regulated activities.


Purchase Online Rights and Permissions

  
  



Article Details

Volume: 3
First Page: 1
Page Count: 1
DOI: 10.2174/2211550103666140701191009
Advertisement

Related Journals




Webmaster Contact: urooj@benthamscience.org Copyright © 2014 Bentham Science