Interactive Effect of Agitation Speed and Aeration Rate on Heat Stable β-Carotene Production from Mucor Azygosporus using Deprotenized Waste whey Filtrate in Stirred Tank Reactor
Wamik Azmi, Meenu Thakur, Azhar Javed and Neetika ThakurAffiliation:
Department of Biotechnology Himachal Pradesh University, Summer Hill, Shimla - 171005, India.
AbstractInteractive effects of aeration rate and agitation speed on β-carotene production by Mucor azygosporus using waste whey filtrate in a laboratory fermenter were studied. The maximum β-carotene concentration (1045 µg/g dcw) was obtained at low agitation speed (150 rpm) and moderate aeration rate (1.0 vvm) after 72 h of incubation with a biomass of 7.9 g/L. The agitation and aeration provided better homogeneity in the culture medium with greater availability of nutrients and oxygen leading to a 2.8-fold increase in β-carotene production from that in the shake flask.
β-carotene, M. azygosporus, fermentation, whey filtrate, stirred tank reactor
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