Discrimination of Chemical Profiles of Some Bacterial Species by Analyzing Culture Headspace Air Samples Using TD-GC/MS

ISSN: 1875-6727 (Online)
ISSN: 1573-4110 (Print)

Volume 13, 6 Issues, 2017

Download PDF Flyer

Current Analytical Chemistry

This journal supports open access

Aims & ScopeAbstracted/Indexed in

Submit Abstracts Online Submit Manuscripts Online

Samuel Achilefu
Washington University
St. Louis, MO
Email: cac@benthamscience.org

View Full Editorial Board

Subscribe Purchase Articles Order Reprints

Current: 1.238
5 - Year: 1.15

Discrimination of Chemical Profiles of Some Bacterial Species by Analyzing Culture Headspace Air Samples Using TD-GC/MS

Current Analytical Chemistry, 10(4): 488-497.

Author(s): Ileana-Andreea Rat iu, Victor Boco s-Bin tin tan, Matthew Turner, Victor-Hugo Moll and Charles Lawrence Paul Thomas.

Affiliation: Faculty of Environmental Science and Engineering, Babe s-Bolyai University of Cluj-Napoca, Str. Fantanele nr. 30, 400294 Cluj-Napoca, Romania.


In an attempt to discriminate and identify several bacterial species, headspace air samples above a series of bacterial cultures were interrogated using a TD-GC/MS system. Three microorganism strains with relatively low pathogenic character – Escherichia coli, Bacillus subtilis and Staphylococcus aureus – were investigated during a period of 72 hours from the inoculation. Air samples with a volume of 1 L each were collected from the headspace above the bacterial cultures in agar growth medium of a 5 cm3 volume kept in 30 cm3 glass vials, in Tenax-Carbograph desorption tubes, by using a custom-built sampling unit based upon a portable air sampling pump. One sample was taken for each of the 10 different cultures of a bacterial strain, at 24, 48 and 72 hours from the incubation. The adsorption tubes were analyzed using the TD-GC/MS system and the resulting datasets – chromatograms and associated mass spectra – were used to produce a qualitative insight into the chemical profile of the headspace air above the bacterial cultures. Analysis by TD-GC/MS indicated that the chemical profile of the headspace air samples was complex and changed during the three days monitoring period; also, there appeared to be a series of chemicals specific to individual species of microorganisms.


Microorganisms identification, bacterial chemical profile, TD-GC/MS.

Purchase Online Order Reprints Order Eprints Rights and Permissions

Article Details

Volume: 10
Issue Number: 4
First Page: 488
Last Page: 497
Page Count: 10
DOI: 10.2174/157341101004140701105219
Price: $58
2nd Good Laboratory Practice Compliance Monitoring Summit

Related Journals

Webmaster Contact: urooj@benthamscience.org Copyright © 2016 Bentham Science