Development and Validation of a Capillary Zone Electrophoresis Method for the Determination of Calcium in Composite Biomaterials
Juan P. Cattalini, Javier Garcia, Viviana S. Mourino and Silvia E. LucangioliAffiliation:
Department of Pharmaceutical Technology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, 956 Junin 6th
Floor, Buenos Aires CP1113, Argentina. National Research Council (CONICET), Argentina.
AbstractThis work presents the development and validation of a capillary zone electrophoresis method for calcium ions release quantitation from composite biomaterials with potential application in bone tissue engineering. Calcium ions quantitation was made using a fused silica capillary (40 cm, 75 m ID) and background electrolyte containing 5 mM imidazole, 6 mM α-hydroxyisobutyric acid, 1 mM 1,4,7,10,13,16-hexaoxacyclo-octadecane and 20 % w/v methanol, at pH 4.5. Indirect UV-detection mode at 214 nm was performed. The separations were achieved using normal polarity at 6 kV, a cartridge temperature of 25 °C and 0.5 psi for 5 s for sample introduction. Parameters of validation such as specificity, linearity, limit of detection and quantitation, accuracy, precision and robustness were evaluated according to the International Conference on Harmonization guidelines. The new developed method resulted to be suitable for calcium determination from composite biomaterials for potential application in bone tissue engineering.
Calcium quantitation, capillary zone electrophoresis, composite materials, indirect UV, ions release, validation.
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